Effects of Hb C, D, E, and S traits on measurement of Hb A1c

Sebia

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Issue 02 | July 2016

ESL Biosciences, in partnership with Sebia, offer the most comprehensive range of electrophoresis diagnostic technologies

Effects of haemoglobin C, D, E, and S traits on measurement of HbA1c by twelve methods
(C. Rohlfing et al, 2016)

Haemoglobin interference with HbA1c results

HbA1c is a well-known method of diagnosing diabetes. Common haemoglobin variants have been shown to interfere with HbA1c results from some assay methods. Clinically significant interferences may result in either over- or under-treatment. With several common haemoglobin variants, it is important to know which methods show interference from one or more of these variants.

The Sebia Capillarys 3 Tera and Capillarys 2 Flex-Piercing instruments were two of the assay methods used in this research, and were the only two instruments using capillary electrophoresis for HbA1c measurement. They performed very well in the test, showing NO clinically significant interference from any of the variants detected. Several other HbA1c assay methods showed clinically significant interference, which could adversely affect patient care.

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Capillarys 3 Tera

The Capillarys 3 Tera was shown to have the best performance in the presence of the most common haemoglobin variants. It showed a minor variation in HbAE, and no significant variation in any of the other haemoglobin variants. There were no clinically significant interferences.

"The D-100, Variant II NU, and Variant II Turbo 2.0 showed statistically significant differences for HbAS, HbAE, and HBAD."

"Box-plots summarising the absolute differences (% HbA1c and mmol/mol HbA1c) between each test method and the comparison method for HbAA, HbAC, HbAD, HbAE, and HbAS. The horizontal line in each box is the median difference between the test and comparison methods. The upper and lower limits of each box correspond to the 25th and 75th percentile of the differences, respectively. The highest and lowest horizontal bars represent the minimum and maximum differences between the test and comparison methods. Differences from HbAA that are statistically significant are indicated (#) below each bar where appropriate; clinically significant differences are indicated (*) above each bar where appropriate."

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