The use of anti-dsDNA RIA (the Farr assay) has its downside in the disposal of radioactive materials. Thus a non-radioactive alternative method that produces high specificity and sensitivity in detecting anti-dsDNA is much needed.
EUROIMMUN anti-dsDNA-NcX ELISA differs from conventional anti-dsDNA ELISA systems by using highly purified nucleosome as the linkage molecule between microplate surface and dsDNA molecules. Nucleosome molecules not only provide strong affinity to pure dsDNA, but antibodies against nucleosomes are also an exclusive marker for SLE. Using nucleosome as the linking molecule in Euroimmun anti-dsDNA-NcX ELISA reduces non-specific binding, and produces results that are high in sensitivity and specificity for SLE.