The CoolLED pE-4000 was designed specifically with microscopy core facilities and high end researchers in mind.
 
For these groups, the biggest challenge is deciding how to serve multiple microscope users with varying requirements now and in the future, where fluorophore usage and therefore excitation wavelengths may yet be unknown.
 
Whilst other possible solutions boast up to 6 or 7 wavelengths and generally cover the traditional visible range, the pE-4000 is delivered with 16 wavelengths from 365nm to 770nm. This means that there is never a compromise when selecting the wavelength to use and people are prepared for the increasing move to NIR dyes.
 
With its unique patent pending wavelength grouping concept there is always a combination suitable for samples and filter sets whether it's something "everyday" such as DAPI/FITC/TRITC/Cy5 or something more exotic such as CFP/YFP/RFP/Cy7.
 
16 wavelengths also brings the option to tune the approach. For example, even if only one GFP filter set exists in the microscope, microscopists can optimise for brightness, contrast or other goals as needed, by simply using the different relevant wavelengths in the pE-4000 (460, 470, or 490)
 
The CoolLED pE-4000 is more than just a light source…